Evidence Supporting a Zoonotic Origin of Human Coronavirus Strain NL63
Identifieur interne : 001F87 ( Main/Exploration ); précédent : 001F86; suivant : 001F88Evidence Supporting a Zoonotic Origin of Human Coronavirus Strain NL63
Auteurs : Jeremy Huynh [États-Unis] ; SHIMENA LI [États-Unis] ; Boyd Yount [États-Unis] ; Alexander Smith [États-Unis] ; Leslie Sturges [États-Unis] ; John C. Olsen [États-Unis] ; Juliet Nagel [États-Unis] ; Joshua B. Johnson [États-Unis] ; Sudhakar Agnihothram [États-Unis] ; J. Edward Gates [États-Unis] ; Matthew B. Frieman [États-Unis] ; Ralph S. Baric [États-Unis] ; Eric F. Donaldson [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2012.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Biologie informatique, Chiroptera (virologie), Coronavirus humain NL63 (génétique), Données de séquences moléculaires, Fonctions de vraisemblance, Fèces (virologie), Humains, Infections à coronavirus (transmission), Lignée cellulaire, Maryland, Modèles génétiques, Phylogénie, Réplication virale (physiologie), Séquence nucléotidique, Technique d'immunofluorescence, Technique de Western, Théorème de Bayes, Zoonoses (virologie), Évolution moléculaire.
- MESH :
- génétique : Coronavirus humain NL63.
- physiologie : Réplication virale.
- virologie : Chiroptera, Fèces, Zoonoses.
- Pascal (Inist)
- Analyse de séquence d'ADN, Animaux, Biologie informatique, Données de séquences moléculaires, Fonctions de vraisemblance, Homme, Coronavirus, Humains, Infections à coronavirus, Lignée cellulaire, Maryland, Modèles génétiques, Phylogénie, Séquence nucléotidique, Technique d'immunofluorescence, Technique de Western, Théorème de Bayes, Zoonose, Origine, Souche, Évolution moléculaire.
- Wicri :
English descriptors
- KwdEn :
- Animals, Base Sequence, Bayes Theorem, Blotting, Western, Cell Line, Chiroptera (virology), Computational Biology, Coronavirus, Coronavirus Infections (transmission), Coronavirus NL63, Human (genetics), Evolution, Molecular, Feces (virology), Fluorescent Antibody Technique, Human, Humans, Likelihood Functions, Maryland, Models, Genetic, Molecular Sequence Data, Origin, Phylogeny, Sequence Analysis, DNA, Strain, Virus Replication (physiology), Zoonoses (virology), Zoonosis.
- MESH :
- geographic : Maryland.
- genetics : Coronavirus NL63, Human.
- physiology : Virus Replication.
- transmission : Coronavirus Infections.
- virology : Chiroptera, Feces, Zoonoses.
- Animals, Base Sequence, Bayes Theorem, Blotting, Western, Cell Line, Computational Biology, Evolution, Molecular, Fluorescent Antibody Technique, Humans, Likelihood Functions, Models, Genetic, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA.
Abstract
The relationship between bats and coronaviruses (CoVs) has received considerable attention since the severe acute respiratory syndrome (SARS)-like CoV was identified in the Chinese horseshoe bat (Rhinolophidae) in 2005. Since then, several bats throughout the world have been shown to shed CoV sequences, and presumably CoVs, in the feces; however, no bat CoVs have been isolated from nature. Moreover, there are very few bat cell lines or reagents available for investigating CoV replication in bat cells or for isolating bat CoVs adapted to specific bat species. Here, we show by molecular clock analysis that alphacoronavirus (α-CoV) sequences derived from the North American tricolored bat (Perimyotis subflavus) are predicted to share common ancestry with human CoV (HCoV)-NL63, with the most recent common ancestor between these viruses occurring approximately 563 to 822 years ago. Further, we developed immortalized bat cell lines from the lungs of this bat species to determine if these cells were capable of supporting infection with HCoVs. While SARS-CoV, mouse-adapted SARS-CoV (MA15), and chimeric SARS-CoVs bearing the spike genes of early human strains replicated inefficiently, HCoV-NL63 replicated for multiple passages in the immortalized lung cells from this bat species. These observations support the hypothesis that human CoVs are capable of establishing zoonotic-reverse zoonotic transmission cycles that may allow some CoVs to readily circulate and exchange genetic material between strains found in bats and other mammals, including humans.
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Le document en format XML
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Carolina</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Shimena Li" sort="Shimena Li" uniqKey="Shimena Li" last="Shimena Li">SHIMENA LI</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Epidemiology, University of North Carolina</s1><s2>Chapel Hill, North Carolina</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Epidemiology, University of North Carolina</s1><s2>Chapel Hill, North Carolina</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Smith, Alexander" sort="Smith, Alexander" uniqKey="Smith A" first="Alexander" last="Smith">Alexander Smith</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Epidemiology, University of North Carolina</s1><s2>Chapel Hill, North Carolina</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Sturges, Leslie" sort="Sturges, Leslie" uniqKey="Sturges L" first="Leslie" last="Sturges">Leslie Sturges</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>The Save Lucy Campaign</s1><s2>Annandale, Virginia</s2><s3>USA</s3><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Olsen, John C" sort="Olsen, John C" uniqKey="Olsen J" first="John C." last="Olsen">John C. Olsen</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina</s1><s2>Chapel Hill, North Carolina</s2><s3>USA</s3><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Nagel, Juliet" sort="Nagel, Juliet" uniqKey="Nagel J" first="Juliet" last="Nagel">Juliet Nagel</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>University of Maryland Center for Environmental Science, Appalachian Laboratory</s1><s2>Frostburg, Maryland</s2><s3>USA</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Johnson, Joshua B" sort="Johnson, Joshua B" uniqKey="Johnson J" first="Joshua B." last="Johnson">Joshua B. Johnson</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>University of Maryland Center for Environmental Science, Appalachian Laboratory</s1><s2>Frostburg, Maryland</s2><s3>USA</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Agnihothram, Sudhakar" sort="Agnihothram, Sudhakar" uniqKey="Agnihothram S" first="Sudhakar" last="Agnihothram">Sudhakar Agnihothram</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Epidemiology, University of North Carolina</s1><s2>Chapel Hill, North Carolina</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Gates, J Edward" sort="Gates, J Edward" uniqKey="Gates J" first="J. Edward" last="Gates">J. Edward Gates</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>University of Maryland Center for Environmental Science, Appalachian Laboratory</s1><s2>Frostburg, Maryland</s2><s3>USA</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Frieman, Matthew B" sort="Frieman, Matthew B" uniqKey="Frieman M" first="Matthew B." last="Frieman">Matthew B. Frieman</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Department of Microbiology and Immunology, University of Maryland at Baltimore</s1><s2>Baltimore, Maryland</s2><s3>USA</s3><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Epidemiology, University of North Carolina</s1><s2>Chapel Hill, North Carolina</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Donaldson, Eric F" sort="Donaldson, Eric F" uniqKey="Donaldson E" first="Eric F." last="Donaldson">Eric F. Donaldson</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Epidemiology, University of North Carolina</s1><s2>Chapel Hill, North Carolina</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Bayes Theorem</term><term>Blotting, Western</term><term>Cell Line</term><term>Chiroptera (virology)</term><term>Computational Biology</term><term>Coronavirus</term><term>Coronavirus Infections (transmission)</term><term>Coronavirus NL63, Human (genetics)</term><term>Evolution, Molecular</term><term>Feces (virology)</term><term>Fluorescent Antibody Technique</term><term>Human</term><term>Humans</term><term>Likelihood Functions</term><term>Maryland</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Origin</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Strain</term><term>Virus Replication (physiology)</term><term>Zoonoses (virology)</term><term>Zoonosis</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Biologie informatique</term><term>Chiroptera (virologie)</term><term>Coronavirus humain NL63 (génétique)</term><term>Données de séquences moléculaires</term><term>Fonctions de vraisemblance</term><term>Fèces (virologie)</term><term>Humains</term><term>Infections à coronavirus (transmission)</term><term>Lignée cellulaire</term><term>Maryland</term><term>Modèles génétiques</term><term>Phylogénie</term><term>Réplication virale (physiologie)</term><term>Séquence nucléotidique</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Théorème de Bayes</term><term>Zoonoses (virologie)</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Maryland</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus NL63, Human</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus humain NL63</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Réplication virale</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Fèces</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Feces</term><term>Zoonoses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Bayes Theorem</term><term>Blotting, Western</term><term>Cell Line</term><term>Computational Biology</term><term>Evolution, Molecular</term><term>Fluorescent Antibody Technique</term><term>Humans</term><term>Likelihood Functions</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Biologie informatique</term><term>Données de séquences moléculaires</term><term>Fonctions de vraisemblance</term><term>Homme</term><term>Coronavirus</term><term>Humains</term><term>Infections à coronavirus</term><term>Lignée cellulaire</term><term>Maryland</term><term>Modèles génétiques</term><term>Phylogénie</term><term>Séquence nucléotidique</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Théorème de Bayes</term><term>Zoonose</term><term>Origine</term><term>Souche</term><term>Évolution moléculaire</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Zoonose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The relationship between bats and coronaviruses (CoVs) has received considerable attention since the severe acute respiratory syndrome (SARS)-like CoV was identified in the Chinese horseshoe bat (Rhinolophidae) in 2005. Since then, several bats throughout the world have been shown to shed CoV sequences, and presumably CoVs, in the feces; however, no bat CoVs have been isolated from nature. Moreover, there are very few bat cell lines or reagents available for investigating CoV replication in bat cells or for isolating bat CoVs adapted to specific bat species. Here, we show by molecular clock analysis that alphacoronavirus (α-CoV) sequences derived from the North American tricolored bat (Perimyotis subflavus) are predicted to share common ancestry with human CoV (HCoV)-NL63, with the most recent common ancestor between these viruses occurring approximately 563 to 822 years ago. Further, we developed immortalized bat cell lines from the lungs of this bat species to determine if these cells were capable of supporting infection with HCoVs. While SARS-CoV, mouse-adapted SARS-CoV (MA15), and chimeric SARS-CoVs bearing the spike genes of early human strains replicated inefficiently, HCoV-NL63 replicated for multiple passages in the immortalized lung cells from this bat species. These observations support the hypothesis that human CoVs are capable of establishing zoonotic-reverse zoonotic transmission cycles that may allow some CoVs to readily circulate and exchange genetic material between strains found in bats and other mammals, including humans.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Maryland</li><li>Virginie</li></region></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Huynh, Jeremy" sort="Huynh, Jeremy" uniqKey="Huynh J" first="Jeremy" last="Huynh">Jeremy Huynh</name></region><name sortKey="Agnihothram, Sudhakar" sort="Agnihothram, Sudhakar" uniqKey="Agnihothram S" first="Sudhakar" last="Agnihothram">Sudhakar Agnihothram</name><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><name sortKey="Donaldson, Eric F" sort="Donaldson, Eric F" uniqKey="Donaldson E" first="Eric F." last="Donaldson">Eric F. Donaldson</name><name sortKey="Frieman, Matthew B" sort="Frieman, Matthew B" uniqKey="Frieman M" first="Matthew B." last="Frieman">Matthew B. Frieman</name><name sortKey="Gates, J Edward" sort="Gates, J Edward" uniqKey="Gates J" first="J. Edward" last="Gates">J. Edward Gates</name><name sortKey="Johnson, Joshua B" sort="Johnson, Joshua B" uniqKey="Johnson J" first="Joshua B." last="Johnson">Joshua B. Johnson</name><name sortKey="Nagel, Juliet" sort="Nagel, Juliet" uniqKey="Nagel J" first="Juliet" last="Nagel">Juliet Nagel</name><name sortKey="Olsen, John C" sort="Olsen, John C" uniqKey="Olsen J" first="John C." last="Olsen">John C. Olsen</name><name sortKey="Shimena Li" sort="Shimena Li" uniqKey="Shimena Li" last="Shimena Li">SHIMENA LI</name><name sortKey="Smith, Alexander" sort="Smith, Alexander" uniqKey="Smith A" first="Alexander" last="Smith">Alexander Smith</name><name sortKey="Sturges, Leslie" sort="Sturges, Leslie" uniqKey="Sturges L" first="Leslie" last="Sturges">Leslie Sturges</name><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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